An experiment to transform dna found in the bacteria e coli

The characteristics of bacteria are altered from an external source to allow them to express a new trait, in this case antibiotic resistance. In is experiment foreign DNA is inserted into Escherichia coli in order to alter its phenotype. The goal of the experiment is to transform E. The bacteria are transformed by a combination of calcium chloride and heat shock.

An experiment to transform dna found in the bacteria e coli

The plate was a lawn LB - Many colonies were found where the plate was streaked with the E.


Amp - No colonies were found Discussion Our results were not as great as we had anticipated, and struggled to find colonies of E. Coli in the LB - plate. If anything, the colonies on the LB - plasmid were too small to see with the naked eye. We took the LBAmp - petri dish into a dark closet to observe the bioluminescence of the colonies.

However, only one tiny colony could be spotted at the edge of our dish. This experiment was primarily for the purpose of growing E. Coli bacteria, but in the process, many more strands of bacteria were bound to be present since it is inevitable.

The phenotype of the transformed colonies allows us to understand the ability for E. Coli bacteria to transform DNA in different environments.


The transformations can be influenced by both the temperature and moisture of the environment, as well as the charge of the DNA and the presence of ampicillin. Conclusion The purpose of this lab is to understand how transformation occurs, as well as the biological results and consequences that come from transformation.

Several changes could have been made to this lab so that the results were more effective. We also should have made sure to spread the bacteria thoroughly around the surface of the bacteria.

We successfully predicted that the transformed bacteria on the ampicillin plate would be luminescent, that no untransformed bacteria would grow on an ampicillin plate, and that untransformed bacteria would grow on an ampicillin-free plate but that it would not be luminescent.Genetic transformation of plants and other organisms does occur naturally.

Bacteria and viruses can move DNA (or RNA) into an organism and cause profound changes. Examples are Agrobacterium tumefaciens (for plants) and HIV (for Humans).

Transformation (genetics) - Wikipedia

The bacterium you will be transforming,, lives in the human gut and is a. 1. INTRODUCTION - OVERVIEW - Biology as a science deals with the origin, history, process, and physical characteristics, of plants and animals: it includes botany, and zoology.

A study of biology includes the study of the chemical basis of living organisms, related sciences include microbiology and organic chemistry.

• Use a plasmid vector to transform bacteria with genes for Green Fluorescent Protein (GFP) and antibiotic resistance in a controlled experiment. • Use the heat shock method of transforming E. coli.

An experiment to transform dna found in the bacteria e coli

In this experiment bacterium Escherichia coli (E. coli) will be used since it is a single cell organism and a single celled organism should be more easily transformed due to the fact that there is only one cell to transform and it contains all of the DNA that is needed and it needs to take up the new gene.

Mar 16,  · In this experiment, we heat-shocked our E. coli to make it more “competent.” To be competent means that the bacteria’s cell membrane is altered in .

The purpose of this experiment was to explore how genetic information is transferred between organisms by changing the genetic information of E. coli, determine the relationship between environment and gene expression in E. coli, and to determine the transformation efficiency of the cells that were transformed.

Activity 4: Transformation of E. coli using green fluorescent protein